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(NRP-279) Expression of GFP in a Kenyan Amaranth Species

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Expression of GFP in a Kenyan Amaranth Species
Danny Ward, John Innes Centre

This image was taken at the AfriPlantSci19 summer school at Pwani University, Kilifi, Kenya. The summer school is an initiative started by the John Innes Centre(JIC) and was run by JIC staff and PhD students on internship placements in Africa. The image was generated in a laboratory practical session led by Prof. Wendy Harwood from the JIC. Students were learning how DNA can be prepared and introduced into a plant host for the plants genetic machinery to translate the new DNA code and produce novel products for a short period of time (transiently). In this case, the DNA encoded Green Fluorescent Protein (GFP) which is used as a marker to visualise that the process has been successful. To the best of our knowledge this is a world first as this species (Amarathus hybridus) has never been tried before for transient expression of GFP. This work is being repeated with the aim for it to be published as a single figure paper, the manuscript of which was a combined group effort from all participants and facilitators of the summer school.

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