Delayed Fluorescence in Arabidopsis
Hannah Rees, Earlham Institute
Circadian clocks are inbuilt time-keepers which allow plants to anticipate daily changes in light and temperature. At the Earlham Institute on the Norwich Research Park we are studying circadian rhythms in Arabidopsis thaliana using delayed fluorescence (DF) imaging. 96-well plates contain around 20 seedlings which are pseudo-coloured for their delayed fluorescence intensity. Plates are imaged within a purposely built dark room at the Earlham Institute using CCD-cameras capable of long exposure times. Photos are taken every hour over the course of a week and help scientists to measure the natural circadian rhythms of the plants. DF intensity levels oscillate with an approximately 24h rhythm even under constant light and temperature. Imaging plants like this allows us to identify clock-gene mutations and natural variation in circadian characteristics.